Studies on the lipolytic activity of sonicated enzymes from Yarrowia lipolytica
نویسندگان
چکیده
منابع مشابه
Effect of Oils Extracted from Plant Seeds on the Growth and Lipolytic Activity of Yarrowia lipolytica Yeast
This study was aimed at evaluating the capability of Yarrowia lipolytica W29 for the synthesis of lipolytic enzymes in a medium containing plant oils from non-conventional sources with some components displaying bioactivity. Oils from almond, hazelnut, and coriander seeds were obtained by using n-hexane (Soxhlet method) and a chloroform/methanol mixture of solvents (Folch method), and their eff...
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Yarrowia lipolytica is a very important yeast because many strains from this yeast are able to produce the extracelular lipases. Cold active lipase is one of the important and widely used enzymes whose spectrum of applications has widened in many industries such as in detergent formulations, food industry, leather processing, environmental bioremediations, and fine chemical synthesis as well as...
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To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to bre...
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15 صفحه اولImproving the thermostability of lipase Lip2 from Yarrowia lipolytica.
Yarrowia lipolytica lipase Lip2 (YlLip2) is a highly versatile biocatalyst. However, its practical use is often hampered by its low stability. Here three complementary protein engineering strategies were used to improve the thermostability of this enzyme. The first strategy was error-prone PCR based directed evolution, which resulted in a YlLip2 variant with a 2.5-fold longer half-life of therm...
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ژورنال
عنوان ژورنال: Ultrasonics Sonochemistry
سال: 2012
ISSN: 1350-4177
DOI: 10.1016/j.ultsonch.2011.06.015